Uji Potensi Senyawa Antimikroba pada Tanaman secara Difusi Sumuran dan Difusi Paper Disk

Abstract

Antimicrobials are antifunctional antibiotics that cause some microbes, especially pathogenic microbes to become resistant. Thus, the initial interaction between peptides and the cell membrane binds to intracellular molecules, resulting in inhibition of sex wall biosynthesis and synthesis of DNA, RNA and protein. The antimicrobial potency test can be carried out using two methods, namely diffusion and dilution. Diffusion method, where the working principle of this diffusion method is the diffusion of the antimicrobial compound into the solid media where the test microbes have been inoculated. Making wells is done by placing wells in the base layer, the antimicrobial compound will diffuse radially in all directions including towards the microorganisms growing on the surface of the agar media. The greatest inhibition in the well-diffusion method using natural antibiotics was galangal with a clear zone diameter of 2 cm. The biggest inhibition in the well-diffusion method using synthetic antibiotics was the antibiotic with a concentration of 6.25 mg. Whereas in the paper disk diffusion method the highest clear zone diameter was synthetic antibiotics with a concentration of 12.5 mg. In practice, there is a clear zone which is used as an indicator that an antibiotic compound has succeeded in inhibiting the growth of a microorganism. The inhibition zone formed is because the compound is active in inhibiting and has a role as an inhibitor for microorganisms. The diameter of the inhibition zone is an indication of the sensitivity of microbes to antibiotics. The wider the diameter of the inhibition, the more sensitive the bacteria. To test the concentration of antibiotic compounds, MIC (Minimum Inhibitory Concentration).