Uji Potensi Senyawa Antimikroba pada Daun Sirih Hijau (Piper Batle) secara Difusi Sumuran dan Difusi Paper Disk

Abstract

Bacteria are microscopic organisms that generally consist of a single cell and do not have a cell nucleus membrane. They have an important role in life, including in the food industry. However, some bacteria can also be detrimental, such as causing food spoilage, infection, and disease in humans. Antibacterial test methods are divided into diffusion and dilution. The diffusion method consists of disk, well and trench methods, while the dilution method consists of broth dilution and agar dilution. The main difference between the two is the medium used, where the diffusion method uses a solid medium and the dilution method uses a liquid medium. The method commonly used is the disk diffusion method, but there are also well and trench methods which aim to observe the diameter of the inhibition zone for the tested bacteria. The disc diffusion method is the most commonly used method for measuring antibacterial sensitivity to an antibiotic. This method involves using filter paper discs as containers to contain the antimicrobial substance. Whereas the well method involves making vertical holes in the agar medium which has been inoculated with the test bacteria. The number and location of the holes are adjusted according to the research objectives, and the holes are filled with the sample to be tested. Paper disk test on betel leaves showed that at a concentration of 6.25 mg, an inhibition zone with a diameter of 1.1 cm was visible. At a concentration of 25 mg, there were zones of inhibition with a diameter of 1.3 cm and 1.2 cm. Furthermore, at a concentration of 12.5 mg, inhibition zones with a diameter of 1.5 cm and 1 cm (with uneven distribution) were seen.