Uji Daya Hambat Antimikroba Secara Difusi Sumuran dan Difusi Paper Disk

Abstract

Tests for the inhibition of microorganisms by antimicrobial compounds can be caused by several factors including disturbances in cell wall constituent compounds, increased cell membrane permeability which can cause loss of biotic cell constituent components. The diffusion method is one of the methods often used to test antimicrobial activity. Furthermore, each hole is covered with the test substance and then incubated at room temperature and with the specified time and in accordance with the test microbe. Finally, the presence or absence of an inhibition zone around the hole was observed. This method uses filter paper discs to accommodate the antimicrobial substance, then placed on an agar plate that has been inoculated with the test microbe and then incubated at a time and temperature according to the optimum conditions for the test microbe. The results of this study showed that the average diameter of the inhibition zone for bacteria using the well-diffusion method was 62 mm. The average diameter of the antibiotic inhibition zone using filter paper as a holding medium was 39 mm.